Rev Med UAS
Vol. 13: No. 3. Julio-Septiembre 2023
ISSN 2007-8013

Inducción de tumores mamarios mediante trasplante ortotópico de células 4T1 en ratones BALB/c de diferente línea endogámica parental: estudio piloto

Induction of mammary tumors by orthotopic transplantation of 4T1 cells in BALB/c mice of different parental inbred line: pilot study

Zeirham Loera-Heras1, Maricruz Anaya-Ruiz2, Julieta Gutiérrez-Saldívar3, Humberto Astiazaran-García1,4,*.

  1. Posgrado en Ciencias de la Salud, Universidad de Sonora, Hermosillo, Sonora, México.
  2. Centro de Investigación Biomédica de Oriente (CIBIOR) del Instituto Mexicano del Seguro Social (IMSS), Metepec, Puebla, México.
  3. Julieta Gutiérrez Saldivar, Hermosillo, Sonora, México.
  4. Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora. México.

*Autor de correspondencia: Humberto Astiazaran-García
Centro de Investigación en Alimentación y Desarrollo, A.C.,
Carretera Gustavo Enrique Astiazarán Rosas No. 46 Col La Victoria, C.P. 83304, Hermosillo, Sonora. México.
e-mail: ,


Texto Completo PDF

Recibido 22 de enero 2023, aceptado 15 de agosto 2023

Objetivo. Desarrollar tumores mamarios mediante el trasplante ortotópico de células 4T1 en ratones BALB/c de diferente línea endogámica parental. Material y métodos. Se realizó un estudio experimental, células 4T1 (5x104) fueron trasplantadas en la cuarta almohadilla de grasa mamaria de ratones hembra BALB/c de la línea endogámica A (LE-A n=4) y línea endogámica B (LE-B n=3). El día del trasplante se denominó día inicial (D0), en los días posteriores al trasplante D7, D14, D21 y D28 fue evaluado el crecimiento tumoral utilizando un calibrador electrónico. Estadística con prueba t de student. Resultados. En el D28 ambas líneas endogámicas desarrollan tumores sólidos con un volumen de 1007.9± 335.6 mm3 (LE-A) y en LE-B 994.0±330.7 mm3 (LE-B) siendo significativamente iguales P >0.05. El grado histológico en ambas líneas endogámicas fue de 3 y se observó un aumento del tamaño del bazo con 291,6±44,3 mm2 (LE-A) y con 282±18,8 mm2 (LE-B) siendo significativamente iguales P >0.05. Conclusiones. Ambas líneas endogámicas (A y B) de la cepa BALB/c desarrollaron tumores sólidos de tamaño, apariencia y características histopatológicas similares.
Palabras clave: Cáncer de mama; modelos murinos; trasplante ortotópico; células 4T1; tumores mamarios.

Objective. Develop mammary tumors by orthotopic transplantation of 4T1 cells in mice of the same BALB/c strain but a different parental inbred line. Materials and methods. An experimental study was carried out. 4T1 cells (5x104) were transplanted in the fourth mammary fat pad of female BALB/c mice from inbred line A (IL-A n=4) and inbred line B (IL-B n=3). The day of transplantation is called the first day (D0), on days’ post-transplantation D7, D14, D21 and D28 tumor growth was assessed using an electronic caliper. Statistical design with t student test. Results. On D28 both inbred develop solid tumors with a volume 1007.9± 335.6 mm3 and 994.0±330.7 mm3 no significant differences were observed P>0.05. The histological grade in both inbred lines was 3 and an increase in spleen size 291.6±44.3 mm2 (IL-A) and 282±18.8 mm2 (IL-B) no significant differences were observed P >0.05. Conclusions. Both inbred lines (A and B) of the BALB/c strain developed solid tumors of similar size, appearance, and histopathology.
Key words: Breast cancer; mouse models; Orthotopic transplantation; 4T1 cell; mammary tumors.


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