Rev Med UAS
Vol. 13: No. 3. Julio-Septiembre 2023
ISSN 2007-8013

Inducción de tumores mamarios mediante trasplante ortotópico de células 4T1 en ratones BALB/c de diferente línea endogámica parental: estudio piloto

Induction of mammary tumors by orthotopic transplantation of 4T1 cells in BALB/c mice of different parental inbred line: pilot study

Zeirham Loera-Heras1, Maricruz Anaya-Ruiz2, Julieta Gutiérrez-Saldívar3, Humberto Astiazaran-García1,4,*.

  1. Posgrado en Ciencias de la Salud, Universidad de Sonora, Hermosillo, Sonora, México.
  2. Centro de Investigación Biomédica de Oriente (CIBIOR) del Instituto Mexicano del Seguro Social (IMSS), Metepec, Puebla, México.
  3. Julieta Gutiérrez Saldivar, Hermosillo, Sonora, México.
  4. Centro de Investigación en Alimentación y Desarrollo, A.C., Hermosillo, Sonora. México.

*Autor de correspondencia: Humberto Astiazaran-García
Centro de Investigación en Alimentación y Desarrollo, A.C.,
Carretera Gustavo Enrique Astiazarán Rosas No. 46 Col La Victoria, C.P. 83304, Hermosillo, Sonora. México.
e-mail: hastiazaran@ciad.mx , humberto.astiazaran@unison.mx

DOI http://dx.doi.org/10.28960/revmeduas.2007-8013.v13.n3.003

Texto Completo PDF

Recibido 22 de enero 2023, aceptado 15 de agosto 2023

RESUMEN
Objetivo. Desarrollar tumores mamarios mediante el trasplante ortotópico de células 4T1 en ratones BALB/c de diferente línea endogámica parental. Material y métodos. Se realizó un estudio experimental, células 4T1 (5x104) fueron trasplantadas en la cuarta almohadilla de grasa mamaria de ratones hembra BALB/c de la línea endogámica A (LE-A n=4) y línea endogámica B (LE-B n=3). El día del trasplante se denominó día inicial (D0), en los días posteriores al trasplante D7, D14, D21 y D28 fue evaluado el crecimiento tumoral utilizando un calibrador electrónico. Estadística con prueba t de student. Resultados. En el D28 ambas líneas endogámicas desarrollan tumores sólidos con un volumen de 1007.9± 335.6 mm3 (LE-A) y en LE-B 994.0±330.7 mm3 (LE-B) siendo significativamente iguales P >0.05. El grado histológico en ambas líneas endogámicas fue de 3 y se observó un aumento del tamaño del bazo con 291,6±44,3 mm2 (LE-A) y con 282±18,8 mm2 (LE-B) siendo significativamente iguales P >0.05. Conclusiones. Ambas líneas endogámicas (A y B) de la cepa BALB/c desarrollaron tumores sólidos de tamaño, apariencia y características histopatológicas similares.
Palabras clave: Cáncer de mama; modelos murinos; trasplante ortotópico; células 4T1; tumores mamarios.

ABSTRACT
Objective. Develop mammary tumors by orthotopic transplantation of 4T1 cells in mice of the same BALB/c strain but a different parental inbred line. Materials and methods. An experimental study was carried out. 4T1 cells (5x104) were transplanted in the fourth mammary fat pad of female BALB/c mice from inbred line A (IL-A n=4) and inbred line B (IL-B n=3). The day of transplantation is called the first day (D0), on days’ post-transplantation D7, D14, D21 and D28 tumor growth was assessed using an electronic caliper. Statistical design with t student test. Results. On D28 both inbred develop solid tumors with a volume 1007.9± 335.6 mm3 and 994.0±330.7 mm3 no significant differences were observed P>0.05. The histological grade in both inbred lines was 3 and an increase in spleen size 291.6±44.3 mm2 (IL-A) and 282±18.8 mm2 (IL-B) no significant differences were observed P >0.05. Conclusions. Both inbred lines (A and B) of the BALB/c strain developed solid tumors of similar size, appearance, and histopathology.
Key words: Breast cancer; mouse models; Orthotopic transplantation; 4T1 cell; mammary tumors.

Referencias

  1. Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, et al. Global Cancer Statistics 2020 : GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. CA Cancer J Clin. 2021 2021;0(0):1–41.
  2. Sanchez YLM, Medrano Guzman R. Epidemiología del cáncer de mama [Internet]. [cited 2023 Jan 17]. Available from: https://www.gob.mx/imss/articulos/epidemiologia-del-cancer-de-mama-318014
  3. Korinek V. Animal Modeling in Cancer. Genes (Basel). 2020;11(9):1–4.
  4. National Human Genome Research Institute. MOUSE MODEL [Internet]. [cited 2022 Apr 23]. Available from: https://www.genome.gov/genetics-glossary/Mouse-Model
  5. Liu C, Wu P, Zhang A, Mao X. Advances in Rodent Models for Breast Cancer Formation, Progression, and Therapeutic Testing. Front Oncol. 2021;11(March):1–11.
  6. Park MK, Lee CH, Lee H. Mouse models of breast cancer in preclinical research. Lab Anim Res. 2018;34(4):160–5.
  7. Potter M. History of the BALB / c Family. In: The BALB/c Mouse. Berlin, Heidelberg.: Springe; 1985. p. 1–5.
  8. Pulaski BA, Ostrand‐Rosenberg S. Mouse 4T1 Breast Tumor Model. Curr Protoc Immunol. 2000 Oct;39(1):20.2.1-20.2.16.
  9. Manjunath M, Choudhary B. Triple-negative breast cancer: A run-through of features, classification and current therapies (Review). Oncol Lett. 2021;22(1):1–21.
  10. Miller BE, Roi LD, Howard LM, Miller FR. Quantitative Selectivity of Contact-mediated Intercellular Communication in a Metastatic Mouse Mammary Tumor Line. Cancer Res. 1983;43(9):4102–7.
  11. Tao K, Fang M, Alroy J, Sahagian GG. Imagable 4T1 model for the study of late stage breast cancer. BMC cancer. 2008;8(1), 1-19.
  12. Hunter KW. Mouse models of cancer : does the strain matter ? Nat Rev Cancer. 2012;12(2):144-149.
  13. Reilly KM. The effects of genetic background of mouse models of cancer: friend or foe? Cold Spring Harb Protoc. 2016;2016(3):pdb-top076273.
  14. Nandi S, Bern HA. Relation Between Mammary-Gland Responses to Lactogenic Hormone Combinations and Tumor Susceptibility in Various Strains of mice. J Natl Cancer Instit. 1960;24(4):907–31.
  15. Murphy KL, Grimm SL, Rosen JM. Modeling Mammary Cancer in Mice Conference,. In: Meeting report. Bar Harbor, Maine; 1999. p. 5–8.
  16. Rivera J, Tessarollo L. Genetic background and the dilemma of translating mouse studies to humans. Immunity,. 2008;28(1):1–4.
  17. Garber JC, Wayne R, Bielitzki JT, Clayton LA, Donovan JC, Hendriksen CF, et al. Committee for the Update of the Guide for the Care and Use of Laboratory Animals. eighth. Washington DC, USA: National Academy of Sciences; 2011.
  18. NOM-062-ZOO-1999. Especificaciones técnicas para la producción, cuidado y uso de los animales de laboratorio. 1999.
  19. Cárdenas-Sánchez J, Erazo Valle-Solís AA, Arce-Salinas C, Bargalló-Rocha JE, Bautista-Piña V, Cervantes-Sánchez G, et al. Consenso Mexicano sobre diagnóstico y tratamiento del cáncer mamario. Octava revisión. Colima 2019. Gac Mex Oncol. 2019;18(3):141–231.
  20. Bloom HJG, Richardson WW. Histological grading and prognosis in breast cancer a study of 1409 cases of which 359 have been followed for 15 years. 1957;
  21. INEGI. Estadísticas a propósito del día mundial contra el cáncer [Internet]. Available from: https://www.inegi.org.mx/contenidos/saladeprensa/aproposito/2023/EAP_Cancer.pdf
  22. Zeng L, Li W, Chen C. Breast cancer animal models and applications. Zool Res.2020;41(5):477–94.
  23. Arroyo‐Crespo JJ, Armiñán A, Charbonnier D, Deladriere C, Palomino‐Schätzlein M, Lamas‐Domingo R, et al. Characterization of triple‐negative breast cancer preclinical models provides functional evidence of metastatic progression. Int J Cancer. 2019 Oct;145(8):2267–81.
  24. Xenografts P, Okano M, Oshi M, Butash A, Okano I, Saito K, et al. Orthotopic Implantation Achieves Better Engraftment and Faster Growth Than Subcutaneous Implantation in Breast Cancer. J Mammary Gland Biol Neoplasia. 2020;27–36.
  25. Zhang Y, Zhang GL, Sun X, Cao KX, Ma C, Nan N, et al. Establishment of a murine breast tumor model by subcutaneous or orthotopic implantation. Oncol Lett. 2018;15(5):6233–40.
  26. Davie SA, Maglione JE, Manner CK, Young D, Cardiff RD, et al. Effects of FVB / NJ and C57Bl / 6J strain backgrounds on mammary tumor phenotype in inducible nitric oxide synthase deficient mice. Transgenic Res. 2007;193–201.
  27. Aupperlee MD, Drolet AA, Durairaj S, Wang W, Schwartz RC, Haslam SZ. Strain-Specific Differences in the Mechanisms of Development. Endocrinol. 2009;150(3):1485–94.
  28. duPre’ SA, Hunter KW. Murine mammary carcinoma 4T1 induces a leukemoid reaction with splenomegaly: Association with tumor-derived growth factors. Exp Mol Pathol. 2007;82(1):12–24.
  29. Fitzgibbons PL, Page DL, Weaver D, Thor AD, Allred DC, Clark GM, et al. Prognostic Factors in Breast Cancer College of American Pathologists Consensus. Arch. Pathol. Lab. Med. 1999;124(7),966-978.