Rev Med UAS
Vol. 12: No. 3. Julio-Septiembre 2022
ISSN 2007-8013

Camperol induce un efecto prooxidante y cambios ultraestructurales como mecanismo amebicida

Kaempferol induces prooxidant effect and ultrastructural changes as amoebicidal mechanism

Rosa Adriana Jarillo-Luna1, Juan Manuel Gutiérrez-Meza1, Elizabeth Barbosa-Cabrera1, Gabriela Rebeca Luna-Palencia2, Martha Cecilia Rosales-Hernández3, Mario Nequiz4, Judith Pacheco-Yepez1,*

  1. Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México. México, 11340.
  2. Departamento de Biotecnología y Bioingeniería, Centro de Investigación y Estudios Avanzados del Instituto Politécnico Nacional. Ciudad de México. México, 07360.
  3. Laboratorio de Biofísica y Biocatálisis, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México. México, 11340
  4. Unidad de Investigación en Medicina Experimental, Facultad de Medicina, UNAM, Ciudad de México. México, 04510

* Correspondencia: Judith Pacheco-Yépez
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional
Plan de San Luis y Salvador Díaz Mirón s/n, Col. Santo Tomas, Ciudad de México. México 11340.
Telefono: 52 55 35183193 e-mail: jpachecoy@ipn.mx

DOI http://dx.doi.org/10.28960/revmeduas.2007-8013.v12.n3.005

Texto Completo PDF

Recibido 09 de mayo 2022, aceptado 16 de junio 2022

RESUMEN
Objetivo: Realizar un análisis comparativo in vitro de la actividad anti-amibiana del flavonoide Camperol comparado con la droga de referencia, el Metronidazol y determinar si el mecanismo de daño es oxidativo. --- Metodología: Se aplicaron diferentes concentraciones de Camperol y Metronidazol a trofozoítos de Entamoeba histolytica, analizando mediante microscopia electrónica el daño estructural ocasionado, por métodos colorimétricos el efecto antiamibiano en y sin condiciones de oxigenación, daño al DNA por método fluorométrico, la citotoxicidad de los fármacos en células HeLa por métodos colorimétrico y la producción de anión superóxido mediante resonancia paramagnética electrónica. Resultados: El grado de destrucción amibiana depende del tiempo y concentración del flavonoide. Camperol mostro una actividad antiambiana contra Entamoeba histolytica similar a la del Metronidazol; indujo cambios ultraestructurales en el citoplasma de los trofozoítos de Entamoeba histolytica, y alteración de la membrana celular, indujo daño al DNA, mostro un efecto prooxidante y mantuvo su actividad anti-amibiana en un medio altamente oxigenado. Finalmente, el Camperol no mostro toxicidad contra células HeLa. --- Conclusiones: Los efectos antiamibianos se deben a las características prooxidantes del Camperol, efecto que supera los mecanismos anti-oxidantes de la amiba, sugiriendo que in vivo sería un mejor amebicida que el Metronidazol.
Palabras clave: Entamoeba histolytica, Camperol, anti-amibiana prooxidante, cambios ultraestructurales

Abstract
Objective: Perform a comparative in vitro analysis of the anti-amoebic activity of the flavonoid Camperol compared to the reference drug, Metronidazole and determine if the damage mechanism is oxidative. --- Methodology: Different concentrations of Camperol and Metronidazole were applied to Entamoeba histolytica trophozoites, analyzing by electron microscopy the structural damage caused, by colorimetric methods the anti- amoebic effect with and without oxygenation conditions, the DNA damage by fluorometric method, the cytotoxicity of Camperol in HeLa cells by colorimetric method and the production of superoxide anion by electron paramagnetic resonance. --- Results: The degree of amoebic destruction depends on the time and concentration of the flavonoid. Camperol showed anti-amoebic activity against Entamoeba histolytica similar to Metronidazole; induced ultrastructural changes in the cytoplasm of Entamoeba histolytica trophozoites, and alteration of the cell membrane, induced DNA damage, showed a prooxidant effect and maintained its anti-amoebic activity in a highly oxygenated medium. Finally, Camperol did not show toxicity against HeLa cells. --- Conclusions: The anti-amoebic effects are due to the pro-oxidant characteristics of Camperol, an effect that exceeds the anti-oxidant mechanisms of the amoeba, suggesting that in vivo it would be a better amebicide than Metronidazole.
Keywords: Entamoeba histolytica, kaempferol, prooxidant anti-amoebic, ultrastructural changes,

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