1. Facultad de Enfermería y Nutrición. Universidad Autónoma de San Luis Potosí.
2. Facultad de Ingeniería. Universidad Autónoma de San Luis Potosí.

*Autor de correspondencia: Gallegos-García Verónica
Dirección: Av. Niño Artillero No. 130. Zona Universitaria. C.P. 78240. veronica.gallegos@uaslp.mx

DOI http://dx.doi.org/10.28960/revmeduas.2007-8013.v11.n1.004

Recibido 22 de Octubre 2020, aceptado 26 de Noviembre 2020

RESUMEN
Introducción. Los aceites esenciales tienen una gran cantidad de compuestos que poseen efecto fungicida y en los últimos años se ha considerado su uso como posibles tratamientos que permitan el control de infecciones. Candida albicans es parte de la microbiota y es una levadura patógena de importancia médica que en ciertas patologías como neoplasia intraepitelial cervical (NIC), diversos cánceres y pacientes con el síndrome de inmunodeficiencia humana puede ocasionar infecciones superficiales o sistémicas. --- Objetivo. Evaluar el efecto fungicida de Chrysactinia mexicana A. Gray sobre aislados clínicos de C. albicans de mujeres potosinas con diferentes grados de NIC. --- Material y métodos. Se extrajo el aceite esencial de Chrysactinia mexicana A. Gray por arrastre de vapor. Se utilizó la técnica de microdilución en placa de 96 pozos para conocer la concentración fungistática y fungicida del aceite esencial y el acetato de linalilo. Se realizaron ensayos de 24h, fase estacionaria y logarítmica de crecimiento sobre 11 aislados clínicos de C. albicans de pacientes con NIC y una cepa ATCC. Posteriormente se observó el crecimiento en cajas de Petri con YPD agar por 48h. --- Resultados. Se observó un efecto fungicida sobre las cepas Ca7 y SC5314 de C. albicans con 3.71mg/ml y 4.64mg/ml respectivamente. La Ca7 de C. albicans mostro inhibición del 100% tubo germinal a la concentración de 0.371mg/ml del aceite esencial de C. mexicana. --- Conclusiones. El aceite esencial de C. mexicana tiene efecto fungicida y fungistático en aislados clínicos de pacientes con NIC.
Palabras Clave: Acetato de linalilo, tubo germinal, fungicida, fungistático

ABSTRACT
Introduction. Essential oils have a large number of compounds that have a fungicidal effect and in recent years their use has been considered as a possible treatment that allows infection control. Candida albicans is a part of the microbiota and is a pathogenic yeast of medical importance that in certain pathologies, such as cervical intraepithelial neoplasia (CIN), various cancers, and patients with human immunodeficiency syndrome, can cause superficial or systemic infections. --- Objective. To evaluate the fungicidal effect of Chrysactinia mexicana A. Gray on clinical isolates of C. albicans from female patients in San Luis Potosi with different degrees of CIN. --- Material and Methods. The essential oil of C. Mexicana was extracted by steam entrainment. The 96-well plate microdilution technique was used to determine the fungistatic and fungicidal concentration of the essential oil and linalyl acetate. 24 h, stationary and logarithmic growth phase tests were performed on 11 clinical isolates of C. albicans from patients with CIN and ATCC strain. Subsequently, growth was observed in petri dishes with YPD agar for 48h. --- Results. A fungicidal effect was observed in C. albicans strains Ca7 and SC5314 with 3.71mg/ml and 4.64mg/ml respectively C. albicans Ca7 showing 100% inhibition of the germ tuve at the concentration of 0.37mg/ml of the essential oil of C. mexicana. --- Conclusions. The essential oil of C. mexicana has a fungicidal and fungistatic effect in clinical isolates from patients with CIN.
Keywords:Linalyl acetate, germ tuve, fungicide, fungistatic

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